Clumping factor B (ClfB), a new surface-located fibrinogen-binding adhesin ofStaphylococcus aureus
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منابع مشابه
Identification of the Staphylococcus aureus MSCRAMM clumping factor B (ClfB) binding site in the αC-domain of human fibrinogen
Clumping factor B (ClfB) of Staphylococcus aureus binds to cytokeratin 10 and to fibrinogen. In this study the binding site in human fibrinogen was localized to a short region within the C terminus of the Aalpha-chain. ClfB only bound to the Aalpha-chain of fibrinogen in a ligand-affinity blot and in solid-phase assays with purified recombinant fibrinogen chains. A variant of fibrinogen with wi...
متن کاملFibrinogen-binding protein/clumping factor from Staphylococcus aureus.
The binding of staphylococcal components to fibrinogen was studied. Fibrinogen-binding material from lysed staphylococcal cells or culture supernatants was affinity purified on fibrinogen-Sepharose and analyzed on Western (immuno-) blots by the use of fibrinogen and antifibrinogen antibodies. Two main bands of 87 and 19 kilodaltons (kDa) and a weaker band of 35 kDa bound specifically to fibrino...
متن کاملStructural organization of the fibrinogen-binding region of the clumping factor B MSCRAMM of Staphylococcus aureus.
The clumping factor B (ClfB) of Staphylococcus aureus is a surface protein that binds to fibrinogen (Ni Eidhin, D., Perkins, S., Francois, P., Vaudaux, P., Hook, M., and Foster, T. J., 1998 Mol. Microbiol. 30, 245-257). The ligand-binding activity is located in the approximately 500-residue A-region (residues 44-542), which represents the N-terminal half of the MSCRAMM protein. We now hypothesi...
متن کاملClumping Factor B, a Fibrinogen-Binding MSCRAMM adhesin of Staphylococcus aureus, also Binds to the Tail Region of Type I Cytokeratin 10
The primary habitat of Staphylococcus aureus in man is the moist squamous epithelium of the anterior nares. We showed previously that S.aureus adheres to desquamated epithelial cells and that clumping factor B, a surface located MSCRAMM known for its ability to bind to the α-chain of fibrinogen, is in part responsible (O'Brien, L.M., Walsh, E.J., Massey, R.C., Peacock, S.J. and Foster, T.J., 20...
متن کاملFibrinogen Binding Sites P336 and Y338 of Clumping Factor A Are Crucial for Staphylococcus aureus Virulence
We have earlier shown that clumping factor A (ClfA), a fibrinogen binding surface protein of Staphylococcus aureus, is an important virulence factor in septic arthritis. When two amino acids in the ClfA molecule, P(336) and Y(338), were changed to serine and alanine, respectively, the fibrinogen binding property was lost. ClfAP(336)Y(338) mutants have been constructed in two virulent S. aureus ...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 1998
ISSN: 0950-382X,1365-2958
DOI: 10.1046/j.1365-2958.1998.01050.x